I have this data of D. melanogaster that has the counts of miRNAs at two different time-0 to 1 hour and 6-10 hour of development. The absence of replicates makes it difficult to use any statistical methods available. How can I alternatively use a statistical model to tell us which microRNA expression differences are significantly different? dme-miR-190-3p 53 24.90535963 dme-miR-190-3p 10 dme-miR-190-5p 855 401.7751413 dme-miR-190-5p 130 dme-miR-193-3p 11 5.169036905 dme-miR-193-3p 5 dme-miR-210-3p 17 7.988511581 dme-miR-210-3p 5 dme-miR-210-5p 12 5.638949351 dme-miR-210-5p 3