Is it possible to analysis the differential expression genes/transcripts form RNA-seq without any replications!!!. Actually I am handling the year old plant transcriptome data of different tissue of male and female plant to find DEGs betwen the tissue of male and female plant using the Tuxedo Package suite (HISAT2-Stingtie-Ballgown).

My sample file for ballgown

ids         sex tissue
FB_S6_L001  female  Bud
FL_S14_L001 female  Leaf
FS_S15_L001 female  Stem
FT_S13_L001 female  Tip
MB_S2_L001  male    Bud
ML_S3_L001  male    Leaf
MS_S5_L001  male    Stem
MT_S4_L001  male    Tip

Want to calculate the DEG between:

Bud   M/F
Leaf  M/F
Stem  M/F
Tip   M/F

How i Can proceed further using ballgown