More posts like this
-
RRBS (Reduced Representation Bisulfite Sequencing)
written by sk_24 •I am working with single-end RRBS data, but I do not have information about the restriction enzyme used during library preparation. Trim Galore offers the …
-
How to predict/annotate whether some type strains has a certain type of enzyme?
written by JyiYeung •Hello, While it might be a naive question, but I really need your help if possible. I want to predict whether some type strains (like …
-
How do i figure out whether a recognition site is conserved or not?
written by Mahmoud Reda •I have two sequences corresponding to the 16s rRNA gene for two strains of a certain species. I simulated the restriction digestion of these sequences …
-
How much differential RNA expression is expected between male and female in a random tissue?
written by nvera •Hello. I suspect there is something fishy in my data because of the observed differences between two groups are absurdly lower than expected. To check …
-
Can I use two restriction enzymes to analyze HiC data using HiCExplorer
written by Yogendra Bordiya •Hi My HiC library was prepared using two restriction enzymes. The HiCExplorere tool findRestSites seems to take only one enzyme site as input. Is there …
-
Software to in silico test restriction enzymes for GBS sequencing and similar approaches
written by DenisHi! We are planning some GBS sequencing experiment. We have the whole genome sequence of organism of interest. But we'd like to test in silico …
-
With Python, how can I solve this restriction mapping problem?
written by francois •I'm struggling with the following Python project, which I'll call **multi-digest restriction mapping**. It is originally a bioinformatic problem, but you really do not need …
-
restriction enzyme in REBASE
written by nora •when I used the software pDRAW32 for virtual sequence analysis by restriction enzymes, I observed that he mentioned that there is an ezyme named att1, …
-
How to use fastools digest function to cut the positive strand of DNA exclusively?
written by bright602 •I am currently dealing with some fasta files using fastools to digest the sequence by MboI restriction enzyme. The command I used is as follow …
-
Chromosome Strand, Gene Direction, Restriction Enzyme Recognition
written by Kate •<p>Hello, I want to design my new experiment and I am a little bit confused.</p> <ol> <li>I have genome sequence frm sequencing project</li> <li>In the …