DESeq2 error in design~ code
Hi everyone I need to run Deseq2, but when I try to run this part of command the following error message appears.
Error in DESeqDataSet(se, design = design, ignoreRank) :
design has a single variable, with all samples having the same value.
use instead a design of '~ 1'. estimateSizeFactors, rlog and the VST can then be used
De plus : Warning message:
In DESeqDataSet(se, design = design, ignoreRank) :
1 duplicate rownames were renamed by adding numbers
I am new to R and DESeq2, Please anyone could hepl me to fix this error. Thank you very much
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Kevin Blighe
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Abdou-samad •
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Abdou-samad •
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Please, provide your code. The error says
design has a single variable, with all samples having the same value.. How have you designed your study?Answered on Bioconductor: https://support.bioconductor.org/p/9139829/#9139830