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Beginner in bioinformatics needs some help

Hi, I am new in Bioinformatics, trying to analyze two colour agilent microarray. I have decided to follow this protocol build the expression matrix step by step from GEO raw data I have created a folder that i have named "Targets.txt". Inside I have created folder "SampleFiles" and downstream three subfolders "Array1", "Array2" and "Array3". I want to read those files and I get a message PErmission denied. I know it is super simple but please can anyone help me.

colour two microarray

Hard to answer, we currently have no idea from which data and from which format you start. CEL files? A matrix with intensity values? Some commands would help that generated this error.

My files are in the format: 8M-8 SG11410002_257236320035_S002_GE2_107_Sep09_2_4 I have 8 files in .txt format per array. 3 arrays in total.

Where are you trying to open this file ? R-studio?

Yep, R Studio.

targetinfo <- readTargets('Targets.txt', sep = '\t') Error in file(file, "rt") : cannot open the connection In addition: Warning message: In file(file, "rt") : cannot open file 'Targets.txt': Permission denied

Right click on R-studio and run it as administrator. Hopefully this should solve the problem.

I solved the problem :) Thank you :)

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