Gene with hundreds of counts very similar, is it possible to use it to get DEGs?
Hi, I'm starting in boinfomatics and I'm using Deseq2 for differential expression analysis. I have an RNAseq dataset, where one of the genes I intend to analyze has hundreds of counts ranging from 10 to 12, with a few counts > 9000 (not identified as outliers by the program). I process this data in Deseq2 and get that the gene is differentially expressed across several samples of interest. What can justify these strange counts? Are the results reliable?
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Anderson •
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Can you confirm that you are referring to a single gene that has variable counts across many samples?
That's exactly it