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bcl2fastq ERROR

Hi all, I have a problem. I'm running bcl2fastq software on Ubuntu, but I can't demultiplex anything as the software gives me this error:

 *ERROR: bcl2fastq::common::Exception: 2021-Sep-02 12:12:34: Success (0): /home/Software/bcl2fastq/src/cxx/lib/layout/Layout.cpp(693): Throw in function void bcl2fastq::layout::handleDefaultSample(bool, bool, size_t, bcl2fastq::layout::LaneInfo&, bool&)
Dynamic exception type: boost::exception_detail::clone_impl<bcl2fastq::common::InputDataError>
std::exception::what: Missing a barcode in lane 1*

I don't Understand why... help me. thanks

bcl2fastq
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written by GiV17 • GiV17

please paste your samplesheet

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written by colindaven colindaven

2 answers

Missing a barcode in lane 1

Check your SampleSheet file to make sure that gets addressed.

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written by GenoMax GenoMax

Thanks all, I checked the Sample-Sheet and actually there were 3 commas (,) too many that did not allow the correct reading.

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written by GiV17 • GiV17

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