This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Testing the net differences between 2 treatments, over the control

Hello all,

I have protein data for 2 different treatments and 1 control (3 biological replicas each). I want to find the net differences between the 2 treatment conditions, reducing the control. I am using a T-test for that purpose.

How it would be best to do it? Thank you!

t-test

Be sure to check the literature on standards for the analysis of this type of data. If this is some kind of mass spec or similar I would check whether limma is an option as it is almost certain that this has more power than a naive t-test. What kind of data is this, and how many measurements are in there? Like dozens, or thousands?

Thank you for the help! I will look into the limma option. It is free DDA LC-MS data. I have a few thousand measurements.

0 answers

No answers yet.

Log in to answer this question.