RNAseq only paired ends reads using featurecounts, should I pay attention to count only reads match to one strand?
Dear all,
I have paired ends RNA seq, after clean the reads I only kept paired reads. so after mapping the paired reads to its genome, I used featurecounts to get the reads count of each gene. But I am not sure, should I be careful only count reads matching to one strand?
Thanks for any help and advice.
• 845 views
•
link
written
by
wang-yanfang •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
RNA-seq analysis
written by sk_24 •
-
Using multimaping reads or unique reads on featurecounts?
written by omargmc.tak •
-
how htseq-count counts unstranded RNA-seq data
written by wiscoyogi •
-
How can I use a short sequence as a probe to fish out sequences have the same part out from raw ill…
written by wang-yanfang •
-
two bacteria rna seq mapping using bowtie2 which mode is better end-to-end or local
written by wang-yanfang •
-
How to count the number of reads aligned to rRNA
written by Javad
-
FeatureCounts output does not contain all the genes in the annotation file
written by devbt15 •
-
Paired end reads merged to single end for mapping - should i allow multi-overlap similar to paired …
written by devikaparvathy •
-
featureCounts for paired bam files and dealing with multimapping reads?
written by bioinfo8
-
how to take the subset of fastq reads in one specific chromosome
written by jolin0701-dy
