orthology enrichment
Hello Researchers,
1. Can any one tell me how to do a reciprocal blast?
2a . How do I identify conserved orthologous genes and annotation of specific sequences? and then
2b . How do I map specific sequences on conserved orthologous genes?
I tried taking 3 protein sequences of different species and did normal protein blast with specific e value 1*10^(-6) but I don't know what is reciprocal blast. How do I find evolutionary conserved orthologous genes?
Thank you
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Ram
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isha.lily20 •
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Hi Ram, I tried the same as mentioned exported 3 protein sequences of different species just did normal protein blast
Please google "reciprocal blast" - there are Biostars posts that address the topic. Always search online and try implementing a reliable solution you find on an online forum/paper.
If a
Reciprocal Best Hit(RBH) search is what you want to do, try theeasy-rbhmodule fromMMseqs2.As for your second (and maybe third) questions, dig around in this book perhaps?