Thanks! Looking at the DaPars repo https://github.com/ZhengXia/DaPars, the docs link are broken and the project seems not to be so well maintained. Do you remember when you used this software last? :)
Dear all,
What software do you use to detect alternative polyadenylation from standard short-read RNA-seq?
Thanks, Gregor
4 answers
Found this review for now:
"A survey on identification and quantification of alternative polyadenylation sites from RNA-seq data"
We have successfully used DaPars before. But we've no real reason to suspect its the best, but it found at least some things we believed.
We last used it earlier this year, but that doesn't mean we installed it then.
Looking around, I see their appears to be a DaPars2, which appears to be more actively maintained. https://github.com/3UTR/DaPars2
Nice! Will check it out, didn't see this one, the docs also look to be there, thanks again
Another one I found is QAPA, https://github.com/morrislab/qapa
This one seems updated and recent:
APAlyzer: https://www.bioconductor.org/packages/devel/bioc/vignettes/APAlyzer/inst/doc/APAlyzer.html
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