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clustalw warning: Bisectingkmeans(): Can't split cluster no. xx

I'm trying to run clustalw to align quite a many sequences. This is my command

clustalo -i Bacteria.fsa -o ./Bacteria.msf

but I am keep on getting this warning sign

WARNING: BisectingKmeans(): Can't split cluster no. 5 which has 104 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 143 which has 178 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 223 which has 187 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 163 which has 110 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 10 which has 195 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 76 which has 540 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 1 which has 269 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 365 which has 124 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 304 which has 110 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 370 which has 137 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 419 which has 189 objects any further. Hope it's not too big and doesn't slow things down.

How do I fix this?

Please help me. Thank you!

clustalo clustalomega clustalw
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updated by emma.a • emma.a • written by seok1213neo • seok1213neo

Now I also encounter this problem, may I ask, have you solved this problem? If so, how did you do it

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written by 慧鹏 • 慧鹏

Hi, do you have a solution?

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written by emma.a • emma.a

Please do not add answers unless you're answering the top level question. Instead, use Add Comment or Add Reply as appropriate. I've moved your post to the right location this time, please be more careful in the future.

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written by Ram Ram

Hi, has someone any idea how to "fix" it? Being a WARNING usually doesn't affect the results, but if there is any raccomandation is welcome! :)

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written by emma.a • emma.a

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