Entering edit mode
3.0 years ago
Anna
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0
I'm doing a gene annotation for the pathways project: Sin1 in D.takahashii. I've only been getting one or two hits on BLAST searches, always with e-values of 0. I had two hits for my tblastn, both with one range each. My blastp search for the identified target gene only had one hit, and the Lab Notebook chart expects at least two. I feel like I'm doing something wrong with my searches, but I can't find any errors. Am I overthinking this, or missing a step? https://thegep.org/pathways/ https://thegep.org/lessons/ksandlin-walkthrough-drosophila_pathways/
perhaps the goal is to annotate the missing gene? ;-)
My understanding was that I am annotating Sin1 in D. takahashii using the protein sequence for Sin1 in D. melanogaster. I have a good hit for that on the blastp with e value 0, but the instructions and lab notebook both indicate that there should be multiple hits. I'm not sure if I'm just overthinking this or if I'm missing a step or search parameter? The other picture shows the tblastn results, which also had e value 0 and only one range each. The lab notebook has instructions for many ranges here too...
if you run a blastp against the genome annotation of species A and get one hit, then you run tblastn with your query protein against the genome and it shows 2 hits this likely means there might be a gene missing from the genome annotation result.
I'm working with general concepts here, I have no idea/experience with this exercise in detail.