Hello,

I am wondering if someone could guide me or recommend me some reading material to understand what sort of enrichment (if any) can I expect from a paired-end Illumina TruSeq RNAseq sequencing. I mean, is there a 3' or 5' enrichment that I should be aware of? (as for example 10x does in their 5' or 3' library prep kits).

Also, I would love to start a discussion on how to test this -- For future reference and for different library preparation kits.

Thanks!