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Help with MrBayes parameters

Hi everyone. I have 51 Sequences. When I tried to run the MrBayes with this parameter listed below: it cannot execute. These are the parameters

begin MrBayes;
charset first_pos = 1-721/3
charset second_pos = 2-721/3
charset third_pos = 3-721/3
partition_by_codon = 3: first_pos, second_pos,third_pos;
set partition = by_codon;
lset net=6 rates=invgamma;
mcm ngen = 1000 printfreq = 100 samplefreq=10 nchains=4 savebrlens=yes;
sump burnin = 25;
sumt conformat = simple burnin = 25;
end;

Please help and what could be wrong with these parameters.

mrbayes

it cannot execute

As in the program does not run at all or you get an error. If latter can you add that error to the original post?

You need to load in the alignment. Burn-in is represented as a fraction (0.25), not as a percentage.

Difficult to troubleshoot further without an error message.

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