Detection of bacterial SNP using regular PCR primers
Hi all,
I have two bacterial strains that only differ from several SNPs in their genomes. I am wondering if designing specific primer sequences containing the SNP mutation will be enough to differentiate the two strains using regular PCR?
I have heard of locked nucleic acid oligonucleotides but I would rather use regular PCR if possible.
Thank you in advance for you much appreciated help and advices!
Audrey
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A_heath •
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I doubt sequences differing by only a single SNP allow you to distinguish by PCR alone. If you want to avoid sequencing, would restriction digests be an option for your SNPs of interest?