Problem in a microbial functional gene analysis.
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3.0 years ago

I have analysed mcrA sequences in QIIME2. The mcrA database was extracted from the NCBI website, but the taxonomy barplot assigned few taxa in different ranks. In comparison to other publications, the result was unacceptable and too weird. Then I blasted my DNA sequences in BLASTx then I got more taxa. It sounds DNA database for mcrA was not appropriate. I need to know, based on this status, how can I analyse my mcrA-related DNA sequence with QIIME2 or other methods? What is your suggestion? Thanks

database Protein • 440 views
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