Issues with Centrifuge indexing
Hi! I am very new to bioinformatics and am trying to follow the centrifuge tutorial (https://ccb.jhu.edu/software/centrifuge/manual.shtml#centrifuge-example). I am having issues building an index using Centrifuge build.
I am trying to run this code from the tutorial on Centrifuge 1.0.4 Beta:
centrifuge-build -p 4 --conversion-table seqid2taxid.map \
--taxonomy-tree taxonomy/nodes.dmp --name-table taxonomy/names.dmp \
input-sequences.fna abv
My output looks like this:
Settings:
Output files: "abv.*.cf"
Line rate: 7 (line is 128 bytes)
Lines per side: 1 (side is 128 bytes)
Offset rate: 4 (one in 16)
FTable chars: 10
Strings: unpacked
Local offset rate: 3 (one in 8)
Local fTable chars: 6
Max bucket size: default
Max bucket size, sqrt multiplier: default
Max bucket size, len divisor: 4
Difference-cover sample period: 1024
Endianness: little
Actual local endianness: little
Sanity checking: disabled
Assertions: disabled
Random seed: 0
Sizeofs: void*:8, int:4, long:8, size_t:8
Input files DNA, FASTA:
input-sequences.fna
Reading reference sizes
Time reading reference sizes: 00:10:02
Calculating joined length
Writing header
Reserving space for joined string
Joining reference sequences
Killed
Any help would be greatly appreciated. Thank you!
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Killed usually means that was terminated by the operating system (as opposed to a program crashing out).
So it does sound like it is running out of resources.
Try making a smaller file (a subset of what you have) =, the see if you can run the process all the way.
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Are you running out of memory? How much memory do you have available?
Hi! I don't think I am running out of memory. I've requested 45000 from our server, and the file is only 86 GB. I can request more memory if you think that would help! Thank you!
Is that 45G? Then you should try asking for more.
Hi, I think it's actually 4.5Gb. I've just been given a green light for 320G so I'm going to give that a whack. Thank you so much!