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How To Extract Common Cds Among Huge Data Set?

Hi everyone, I have 50 Ecoli whole genome sequence data set. I already assemble all the genome. Next step i want to extract all the common CDs among all the 50 contig file in a batch mode. Can anyone please suggest me any approach?

cds
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updated by aawitney • aawitney • written by HG HG

as we discussed before, you can do prodigal and follow by cd-hit and counting in clusters

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written by Pavel Senin Pavel Senin

Yes..its one good idea. In fact have started also. Let me compare others approach also. So that i can compare better result.

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written by HG HG

Hi Pavel, After finding the gene and the traslated file how it is posiible to find funtion of each gene ...for example i need in a tabular format ....

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written by HG HG

1 answer

You could try annotating one of the genomes (I would use prokka http://www.vicbioinformatics.com/software.prokka.shtml). Then BLAST each of the annotated genes against all the other sets of contigs and list only the genes which have a significant hit in each genome,

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written by aawitney • aawitney

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