Hi, I think you could use the APIs in KEGG.For example to get the total numbers of genes in hsa00010.The code is written in Perl:

#!/usr/bin/env perl
use SOAP::Lite +trace => [qw(debug)];
my $serv = SOAP::Lite -> service("http://soap.genome.jp/KEGG.wsdl");
my $result = $serv->get_genes_by_pathway('path:hsa00010');
my $count = 0;
foreach (@{$result}){
    print $_,"\n";
    $count += 1;
}
print $count,"\n";

The answer is 65.

KEGG now operates with REST instead of SOAP. Could be done in a feasible way. But I did this:

wget -O http://rest.kegg.jp/link/pathway/hsa >HSA.txt ##Saving the file
cut -d "      " -f 2 HSA.txt | cut -d ":" -f 2 | sort -u | wc -l ## counting the pathways in Human
283 (pathways in Human in KEGG at present)

~Prakki Datta

Yes, this is the correct number of pathways for human in KEGG and it is also the right way to find them.

You are correct. The answer now is 260.

You can also use perl script to do this:

#!/usr/bin/env perl

use SOAP::Lite;
$wsdl = 'http://soap.genome.jp/KEGG.wsdl';

$results = SOAP::Lite
-> service($wsdl)
-> list_pathways("hsa");

my $file_name = "kegg_hsa_pathways.txt";

if (open (OUTFILE, '>'.$file_name))
{
    foreach $path (@{$results}) {
        print OUTFILE "$path->{entry_id}\t$path->{definition}\n";
    }
    close(OUTFILE);

    print "Content writtent to $file_name!"
}
else{
    die "Error: Error occur in  writing to file\n";
}

how one can compare pathway of human and pathogen [e.g.mycoplasma]??

can any one say me or else reply me on chintan_biotech@yahoo.com

If you need their graphs, I generated them in dot format.

https://github.com/haluk/kegg