.Gr Format From Rna-Seq
I'm newby in this field.. I've got data from dRNA-seq which I have to analyse, I'm given a set of files, first a .fa and .gff file - it's simply a genome as I understand and some .gr files for various conditions tested. How this type of data is obtained, and the most interesting to me: how the second coloumn representing coverage can contain non-integer numbers for non-normalized data (I thought coverage reflects number of transcripts and it should be integer)?
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Jelena_bioinf •
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It would help if you posted a snipped of what the .gr files obtained. People will be familiar with gff and fa files, but I haven't a clue what a .gr fule might be.
The GR file are very simple files with two columns. The first column represents the position in the genome, the second column the coverage at that position.
Seems like the best approach to a problem like this is to define the analysis that has been used up to this point. Without that information, it will be quite difficult to proceed with any further analysis or interpretation. The usual source of such information is the person or group that processed the data.