This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Creating A Phylogenetic Tree From A Multiple Sequence Alignment.

Hi all.

I have used muscle (3.8) to perform a multiple sequence alignment on 635 tumor suppressor gene sequences and edited (via perl) the output file so it conforms with FASTA. I would like to generate a phylogenetic tree from the msa file. I am enrolled in an introductory level bioinformatics / scientific computing course at a local community college and this would directly relate to my semester project requirement.

Thanks for the help.

Caitlin

multiple-alignment

Your question does tell us what it is that your having problems with? Do you just want to know which tree building software to use?

Hi.

Yes, please. Being a fledgling bioinformaticist, I am quite eager to learn what tool(s) would be most appropriate for the task described above.

Hi, how did you finish this part? I also have to Create A Phylogenetic Tree From A Multiple Sequence Alignment. It's urgent project. Hope you can read it soon. Thank you.

Hi,

Please do not add answers to existing questions unless you're answering the question. The right thing to do would be to add a comment or open a new question and reference this one. You've opened a new question, so you might just want to edit it and add a reference to this post.

I'm moving your "answer" to a comment now.

2 answers

I think that the following link and references/links within is quite good for discussing the types of tree building approaches, and also mentions some of the software to use for each method as well: http://cshprotocols.cshlp.org/content/2008/4/pdb.ip49.full

While the Cold Spring Harbour Protocols and Current Protocols in Bioinformatics are great for this, they do suffer from the disadvantage of requiring a subscription (hopefully your institution has one). However when these are not an option there are some other choices...

The many phylogenetic analysis tutorials on the web may also be helpful: http://www.google.com/search?q=phylogenetic+analysis+tutorial

One good software especially for windows gui is MEGA 5.1, it allows you to run many of the basic tree-building algorithms: parsimony, neighbor-joining, maximum-likelihood etc. You can then compare your results and see. No guarantee it will work with 400 sequences though http://www.megasoftware.net/

Log in to answer this question.