Blast2Go Doesn'T Work With My Fasta File
Hi all, i downloaded human unigene fasta file from Here (Hs.seq.uniq.gz with .uniqe format), and change it to .fasta by notepad. when i submitted the file to blast2go it doesn't work and give me an error (Undefined error when performing BLAST at NCBI for null. Please check parameters and input data (blastx, blastp, etc.)). i checked my fasta file several times but i couldn't understand what is the problem. a sample of my sequences is :
>gnl|UG|Hs#S50214165
agagcctaagtcacagtagccctgactacagcattcctggagcccaggctcttttccaca
gaggaggaaagagcaggcagcagagaccatggggcccccctcagcctctccccacagaga
atgcatcccctggcaggggcttctgctcacagcctcacttctaaacttctggaacccgcc
caccactgccaagctcactattgaatccatgccgctcagtgtcgcagaggggaaggaggt
gcttctacttgtccacaatctgccccagcatctttttggctacagctggtacaaagggga
aagagtggatggcaacagtctaattgtaggatatgtaataggaactcaacaagctacccc
aggggccgcatacagcggtcgagagacaatatacaccaatgcatccctgctgatccagaa
tgtcacccagaatgacataggattctacaccctacaagtcataaagtcagatcttgtgaa
tgaagaagcaactggacagttccatgtatacccggagctgcccaagccctccatctccag
caacaactccaaccccaaggaggacaaggatgctgtggccttcacctgtgaacctgagat
tcacagcacaacctgcctgtagtgggtaaatggtcagagcctcccgaagaaaatgcccca
ggccttcctgtgggggccgtcgccggcatcgtgaccggggtcctggtcggagtggcgctg
gtggccgcgctggtgtgtttcctgctccttgccaaaactggaaggtaccacagctttttc
ccatccttctcccaccccctaggctgactccaggccaaaagaaaggagaccttgtcctgt
attcagggccaggctctccacggcctcccaagtccccagggatccttccctcttattcca
cggctcctccctcgtcagctcctgaccctgggttgctgcctcaacagcttcctcctctgt
gaatttggcccaattaaggccccacccctgagtgtggccctagttccctcacacagcaaa
gccacagctgcctggagggagggagagaaggagggagggaagtagggagggagggaggga
gggcgtgctctgtacttggtacctcccattgttcattgaatccatccacacagtgagggg
gatttgcaggctccggatcctgggcaccgtcacacaatggggagtggaccagcctggaca
ggctgggtggggtcagtgccccccatggctgatgtggtaaccataggccctgaggtgtga
gatgggagaacctgggcctcagaacagcatgtcctggagcgtcctttgtgtctcttgcca
aggggttgagagagaggacaaggacactgtcccatttcagcatcaccagccctgagtgca
gggtctggccacagcagggacactgagttccaggagaatccaagacacttgcggtcccct
ccgtgtcctgcactaaagcacagagccacg
it doesn't has any problem but i dont know the problem. if possible help me.
best regards
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Mohammad Reza Bakhtiarizadeh
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Mohammad Reza Bakhtiarizadeh
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It looks fine as displayed here, but we can't know for sure without a link to the original file. Don't use Notepad, it's easy to introduce unintended errors when manually editing.
i wanted to upload my fasta file but it isn't possible here. how can i upload my file ?
and also, i can mail this file.
There are places online to share files (e.g. a public Dropbox folder or Google Docs). But I'm wondering why you edited the file, when it is already in fasta format?
i just change suffix of unigene file and convert it to .fasta (the original file is .uniqe) to blast2go identify it. but i am very confused and my works is paused and i cant run blas2go. can i send the fasta file for you and try it? thanks for any help on this matter
I fixed my problem. just Remove the symbol # (or any symbol) from the gene name.