Count Vs Dispersion Plot
I am analyzing my RNA-seq data with cummeRbund. I have four conditions, 5 biological replicates per condition. Why do the count vs dispersion plots look more or less identical for all four conditions? I can see from the cummeRbund vignette that this is supposed to be the case; I just don't know why.
Any insights would be appreciated.
Thanks.
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Sean Davis
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Eric •
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1 answer
The count-vs-dispersion relationship is roughly governed by the biological counting process used to quantify RNA-seq data. You might take a look at papers by Simon Anders and Wolfgang Huber (authors of DEXSeq and DESeq) for some background and details.
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