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What Are Their Meanings In The Raw Data Of Mirna Sequencing Reads ?

The GSM652853ptr01.txt comes from http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM652853 (miRNA sequencing raw reads).

[science@localhost GSE26545RAW]$ head GSM652853ptr_01.txt

8      1   575      886      GGGACTGACCTGAAATGAAGAGAATTCGTATGCCGT
8      1   875      922      GGAGGTAGGAGATTATAAGGATACTGTATGGCCTTC
8      1   277      238      GCAAAGCACACGGCCTGCAGAGTTCGTATGCCGTCT
8      1   328      467      GAGAAAGCTTATAAGAACTGCTAACTAATCGTATGC
8      1   367      122      GGGGTGAAACAGATAAAGACTCTCGTATGCCGTCTT
8      1   39      340      GAAGAGCTTTCAGAATGTCAGGGATTCGTATGGTGT
8      1   359      238      TCGAATGACGTCTTCTGCTTGGAAAAAAAAAAAAAT
8      1   344      175      GAGACAACAGGACAAGGCAACACATATCGTATGCCG
8      1   912      273      GTGTTACAGCAGACTTCAGGACAAGATTCGTATGCC
8      1   519      487      GTACAGTACTGCGATAACTGAATCGTATGCCGTCTT

Could someone tell me what are their meanings of the first four columns? Thank you.

mirna sequencing read
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updated by VS VS • written by zhidong.yuan • zhidong.yuan

1 answer

The first four columns give the details of the location of cluster on the Illumina chip. In short : Lane, Tile, x-coordinate, y-coordinate.

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written by VS VS

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