id sno1 sno2 sno3 sno4 gene1 23.42 23.4 88.8 98.21 gene2 0 0 99.7 95.5 gene3 77.4 100 44.4 65.6 gene4 0 0 0 0 gene5 100 100 100 100 : : gene16000 58.3 33.8 78.8 56.6
I have 16000 rows (which represents each gene id) and columns (from different samples-sno1, sno2,sno3 & sno4) which is given in percentage. I want to compare those four samples:(i)how many of genes occur (i.e 100%) between samples (between sno1 & sno2 and between sno3 & sno4) and in all the samples. Eventhough I reduced data rows containg only 0 (for absent) and 100 (present), I come around 1000 rows. I would like to know, if there is any statistical technique (like normalization) to reduce the data dimension, so that it will be easy for heatmap generation.
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jackuser1979
If you simply want to make a heatmap of genes and samples, you can use variance across samples to rank genes by variation and then choose as many as you like to get the picture you want.
Perhaps your problem is one of visualization, though. If that is the case, try making a larger heatmap (large pdf, for example) or use an interactive viewer such as Java Treeview, TIGR MEV, or Partek.
If you don't care so much about the individual genes (which is what you are suggesting when you invoke dimensionality reduction), you could try PCA, NNMF (non-negative matrix factorization), k-means clustering, self-organizing maps to generate "pseudogenes" that could then be plotted. Note that if you have only four samples in your dataset, such dimensionality reduction techniques may be of limited value.
I like the idea of using variance across sample to rank genes and producing heat map. Could you explain a bit.
Simply calculate the variance for each gene and then choose the N genes with the largest variance. N can be whatever you like.
I have calculated variance for each gene.The maximum value of variance is 3000 and minimum value of variance is 180. I have selected threshold of 2500 value to select list of genes and I plotted the heatmap (the list of genes comes now comes around 50). Is there any criteria in selecting threshold?
I do not know of a general approach that fits all situations.
Through ANOVA test we get variance for each gene. Can I use mean square between groups as threshold (Mean square between group comes around 2500) to filter top ranking genes?.
If you have groups and want to find genes that are different between groups, I suggest doing a statistical test to find differentially-expressed genes. Then plot the genes with the most significant p-values.
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Why do you want to reduce the number of rows or columns? You just want a smaller heatmap? around 1000 rows and 4 dimensions actually seems very manageable.
Yes, I want a smaller heatmap.
I could be completely off base, but maybe you could aggregate by biological function (using the gene ontology). I think it will be difficult to reduce your dataset in an unbiased way.