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How to identify the reads from diffient seq-data is from the same gene?

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First I THINK this reads is from the gene Second I can’t identify if the reads are from the gene? IF we have the same standard to identify where the reads from?(if only one base coincidence?)

rna-seq
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updated by rpolicastro rpolicastro • written by 972050141 • 972050141

It's possible for the reads to overhang from the annotated gene. The TSS or TES could be incompletely annotated, there could be a novel splice isoform, or you're getting a larger 5' or 3' UTR. I think the best evidence would be having numerous reads covering that region.

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written by rpolicastro rpolicastro

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