HI,I'm learning about tNGS(Targeted Next-Generation Sequencing) which is aimed to detect pathogenic microbes these days. I think that learning from a project is a good …

Dear all, I was looking for a good way to calculate conservation scores over columns in an MSA. I usually use Kullback-Leibler-Divergence (kl_divergence) or Shannon …

Dears, There are many people use Jaccard distance to measure the genotyping without any justification why jaccard and not any other distance measure. I searched …

Dears, I really confused between SNP and mutation. SNP can occur more than 1% in each column. If the SNP occurs less than 1% then …

Hi everyone, Recently I am using the R package called 'msa', and I have several questions very confused after getting the result. 1. For my …

I have done a multiple sequence alignment for two proteins with similair sequence, however there are some minor differences which discriminate a cytoplasmic form from …

Hi there, I'm looking for a way to extract pairwise bit scores from a multiple sequence alignment without realigning the sequences. Typical scenario is an …

Hi this is my first post. I am doing a Masters research project which revolves around assessing data quality in phylogenomic analyses. The first thing …

​Hi guys, ​I have a questions concerning multiple sequence alignments (msa) building algorithms. Which algorithm is "best", to construct a multiple sequence alignment from a …

<p>Hi,</p> <p>I have a MSA and identified potentially functional important ones using Residue Conservation Score (calculated using Scorecons). Also I have a representative structure for …