how to filter multi-KEGG_ko mapping after functional anntotion using eggnog-mapper

hi everyone!

I had annotated my gene using "eggnog-mapper" with -m diamond, and got the gene_res.emapper.annotations file but there has one question, when I see the KEGG_ko column. I found, one query gene maybe hit different ko number, like:

544-M.-qc_NODE_1191_length_20492_cov_5.830846_8_1   ko:K05589,ko:K13052
544-M.-qc_NODE_1191_length_20492_cov_5.830846_9_1   ko:K07570,ko:K07571
544-M.-qc_NODE_1191_length_20492_cov_5.830846_10_1  ko:K04075,ko:K15780
544-M.-qc_NODE_1191_length_20492_cov_5.830846_11_1  ko:K00760,ko:K15780
gene "544-M.-qc_NODE_1191_length_20492_cov_5.830846_8_1" mapped on "ko:K05589" and "ko:K13052"

so how can I filter them?

next-gen-sequencing gene

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updated 3.2 years ago by Ram

• written 5.4 years ago by MEITUO •

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