This is a test version of Biostars. For the public version, visit https://www.biostars.org.
protein multiple alignment

In protein multiple alignments some columns have the same colour but contain different amino acids. Why?

alignment

Is this a homework/assignment question?

No, I saw it when I was doing a protein alignment on MEGA with clustal w method.

if you needed to guess, what would you think it means? :)

I think they might share the same physiological properties such as being nonpolar, polar (Having charge or not).

exactly.

see @Joe answer for more details

1 answer

Amino acids can be similar, but not the same (e.g. positively or negatively charged). This has implications for the effect on the structure of the protein when they are substituted. CLUSTAL implements a colourscheme to depict this (as do many other tools).

You can find information about CLUSTAL's colouring online:

http://www.jalview.org/help/html/colourSchemes/clustal.html

Log in to answer this question.