Best way to find orthologous gene between two distant species
Hello I am studying the interaction between parasite (flatworm) and fish. I sequenced a parasite genome, and have annotated the protein genes, I want to find their orthologues in fish, as far as I know, the following two methods may achieve this:
- use OMA or Orthofinder;
- use reciprocal BLAST
But I am not sure which method is the most accurate and if there are other suitable methods.
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Hi,
You may want to try this one https://academic.oup.com/gbe/article/12/12/2258/5918455.
Many thanks, I will have a look at this.