Hello -

I am working on an organism with limited NGS data available and which has some ABI 310 genetic data on NCBI which I would like to include given limited available genomic data. However, I am unsure of what processing steps I need to be able to use this data with other illumina/genome data sources.

My first step with the ABI 310 data was to confirm the encoding typing using FASTQC which gives Sanger/Illumina 1.9. With illumina data, next I would normally do a trimming step followed by mapping the reads to a reference genome and then generating a consensus sequence in fasta format.

Given the data that was generate with ABI 310; can I map that data to a reference genome? Is there anything else I should do - additionally pre-processing, post-processing that I am not aware of for taking this data from fastq to fasta format?

Thank you!

Jenna