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Bioinformatics method to detect nascent RNA from RNA-seq data

Hi all,

I am looking for a bioinformatics tool that is capable to differentiate between spliced and unspliced reads and preferably performs counting of nascent RNAs aligned to different features.

Here no metabolic labeling is performed and it’s just a normal bulk RNA-seq data.

I really appreciate if someone can suggest a tool.

Thanks a lot.

rna-seq nascent rna

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