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How to find sequences of unspliced tRNA genes

Dear community,

I have 2 FASTQ files from 2 samples and I have been working on finding which unspliced tRNAs are present in these 2 files coming from these samples. I am not sure whether an unprocessed tRNA would be visible in a FASTQ file (since I consider that it could be evaluated as an artefact), @swbarnes2 suggested me to obtain sequences of unspliced tRNA genes and do the aligning afterwards.

However, I could find regular tRNA sequences, but I could not see any groups of sequences, specifically belonging to unspliced tRNAs.

Could you suggest me a way to find sequences of unspliced tRNA genes?

Thanks in advance!

trna fastq rna-seq next-gen alignment

Hello nkabo!

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