How To Convert Wig File Format Into Fasta Format ?

Question

[monukmr98]

Hi all I have a WIG file format file for some mutation and I want to map these mutations on the chromosome/gene. How can I do that ? Thanks

Answer 1

[Vikas Bansal]

Hi! Have look at this post. You can convert your wig to bed. Then download refseq genes from UCSC or Ensembl in bed format. Then use bedtools intersect.

[monukmr98]

Thanks for your help. I have converted wig file into bed file and also downloaded bedtools. But have been unable to get bed format for human genes from either of the source you mentioned ..! In ensemble, link for fasta format is given. Thanks

[Vikas Bansal]

If you will go to UCSC tables, select your genome of interest, assembly and then gene and prediction (group), refseq gene track and table and download it as a bed format.