Filtering of low expressed gene in obtained from single cell line
Hello Everyone
I am having two biological replicates obtained from one cell line by RNA-sequencing. The alignment was done using STAR and counts were generated using FeautreCounts. I am trying to see whether a gene is being expressed or not in the given cell line? I am not doing any differential gene analysis. My question is how to remove low expressed gene? Should I remove the genes showing zero counts or filter the genes after the normalization? Is it ok to use filterByExpr() if I don't have to compare between treated and control ? I tried to go through the other post but could not come to conclusions. I am looking forward for your suggestions. Thank you so much
• 962 views
•
link
0 answers
No answers yet.
Log in to answer this question.
This has been discussed extensively and many times before, please use the search function, starting with TPM values of expressed genes