The Niehrs laboratory studies regulation of DNA methylation, which plays important roles in development & disease. We have recently demonstrated a role for long non-coding RNAs and R-loop DNA:RNA hybrids in DNA methylation (Arab et al. 2014; Arab et al. 2019). In an ERC-funded project, we now aim to elucidate the mechanisms of DNA demethylation as well as the role played by long non-coding RNAs and R-loops in its regulation. See also https://www.imb.de/research/niehrs/research

The Project

Candidates will make use of mouse embryonic stem cells (ESCs) to interrogate genome-wide the role of ncRNAs and R-loops in targeting GADD45a and the DNA demethylation machinery in the genome. We will use CRISPR/CAS9 mediated ESC mutants, as well as ChIP-seq, RNA-seq, bisulfite-seq to carry out multi- dimensional data analysis to address:

Where in the genome does demethylation occur? What are the molecular determinants in ncRNA & R-loops involved in targeting? What are the cofactors involved? The project will involve strong interaction with a Computational Biologist

Required Qualifications

• PhD & publications in a relevant field
• Sound experience in molecular biology
• Familiarity with Omics techniques or –analysis, or with ncRNAs or R-loops is advantageous
• Excellent communication skills and good team spirit with the ability to solve problems independently
• Fluent in English (spoken and written)

How to apply

Please see details on the project, information on how to apply, and an online application form at http://www.imb.de/postdocs. IMB is an equal opportunity employer.

The deadline for applications is 28 January 2021. Interviews will take place online. Interview dates will be announced once available.

Application webpage: http://www.imb.de/postdocs