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statitistical question: how can I say if there is a difference in the gene that fall inside/outside an especific region?

Hi community, I have an statistical question to ask you. I have a data set, for a specific time point I have 3 groups: -transcribed genes direction 1 (n =x) -transcribed genes direction 2 (n=y) -transcribed genes direction 3 (n=z)

and I have a list with specific nt of interest (n = 5k)

I've calculate the distances from those nt to the genes and I would like to know if there is any difference in those that are inside or outside the gene, lets say:

  • for transcribed direction 1: 50/x are inside and 600 are at 1kb distance
  • for transcribed genes direction 2 : 500/y are inside and 700 are at 1kb distance -for transcribed genes direction 3: 300/z are inside and 500 are at 1kb distance

what is the best way to show if they are different or not? any statistical test to show it?

Thank you

correlation interdependence gene distance

This looks like a contingency table so you could use a Chi-squared test.

but they are not independent samples, so I'm not sure if chi2 is the best way

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