About Cufflinks
When I use the alignment result from bwa with cufflinks, it has the question:
[16:23:18] Loading reference annotation and sequence. Error (GFaSeqGet): end coordinate (161843) cannot be larger than sequence length 161802 Error (GFaSeqGet): subsequence cannot be larger than 16571 Error getting subseq for CUFF.66138.1 (1..16628)!
And could you please give me some advice?Thank you.
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1 answer
The chr's length is wrong, so the length doesn't map.
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