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When I use the alignment result from bwa with cufflinks, it has the question:

[16:23:18] Loading reference annotation and sequence. Error (GFaSeqGet): end coordinate (161843) cannot be larger than sequence length 161802 Error (GFaSeqGet): subsequence cannot be larger than 16571 Error getting subseq for CUFF.66138.1 (1..16628)!

And could you please give me some advice?Thank you.

sam bwa cufflinks

1 answer

The chr's length is wrong, so the length doesn't map.

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