De novo sequencing homework question, what is the parent peptide mass?
In that example, 129+216+327 does not equal the parent's mass of 492.
My understanding is that during MS, the protein is broken down into peptide masses of varying length of approx. 5 to 20 amino acids. They also lose ions such as water or ammonia like in the example., the experimental spectra I thought would show the masses of these "children" that can be summed up to the parent. But that is not the case. Where am I wrong in my understanding?
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Hello nobu.kim66!
We believe that this post does not fit the main topic of this site.
This is not bioinformatics-related.
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This homework question is from my intro to bioinformatics, comp 4980 class at Thompson Rivers University in Canada. I thought De novo sequencing and mass spectrometry are bioinformatics. Please explain why this question is not bioinformatics related.