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Sequences(Without Ref Genome) For Pathway Analysis?

hi! here is my problem: i have some sequence assembled by trinity ,with raw data and downstream Trinity scripts ,a list of Differencial Expression seqs were collected. i wanna do GO and Kegg analysis with those sequences and i have no idea on how to do it cause there is no reference genome .so can anyone give me some advice or recommand some software to do this? thanks!

pathway go analysis

2 answers

try blast2go http://www.blast2go.com/b2ghome

thanks for your reply,i 've used this software,but but it is too slow to annoted my sequence about 200,000(as background to calculate the Erichment as well as P-value),

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