Sequences(Without Ref Genome) For Pathway Analysis?
hi! here is my problem: i have some sequence assembled by trinity ,with raw data and downstream Trinity scripts ,a list of Differencial Expression seqs were collected. i wanna do GO and Kegg analysis with those sequences and i have no idea on how to do it cause there is no reference genome .so can anyone give me some advice or recommand some software to do this? thanks!
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try blast2go http://www.blast2go.com/b2ghome
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thanks for your reply,i 've used this software,but but it is too slow to annoted my sequence about 200,000(as background to calculate the Erichment as well as P-value),
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