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Multi-group DE scRNA-seq comps without replicates

Wondering if its possible to do multi-group DE comparisons of 10X/Seurat data without replicates, using perhaps just the per-cell counts alone? To compare DE genes across clusters from lets say 2 genotypes and 2 treatments.Thanks.

seurat rna-seq scrna-seq deseq2

1 answer

Yes there are definitely methods for DE comparisons implemented in the commonly used single cell packages; for example, see this discussion (link) for scanpy

Do you know of any good tutorials for doing this in Seurat? Thanks.

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