The use of RNA-Seq to analyse expression
Hi guys,
Two questions with regards to RNA-seq:
(i) How would the use of paired-end sequencing in RNA-Seq experiments help you identify alternative splice variants?
(ii) Also, why is a base-by-base alignment not required to quantify gene expression?
Thank you.
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Are these assignment questions?
No, these are questions from my lecture notes on transcriptomics.