Sequence Duplication levels · Biostar

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Sequence Duplication levels

Hello all,

I have a question regarding duplication levels shown in MultiQC report. The raw data went through HTStream for preprocessing and MultiQC was used to check. In the report I saw that when I ran multiQC on 3 different lanes of the same sample it had about 40% duplication for all three lanes (L001, L002, L003 had 40-41%) but the same sample had about 60% duplication when all 3 lanes were combined. Can anyone explain the reason for this?

Any help is appreciated! Thank you.

rna-seq multiqc duplication pcr-enrichment

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updated 5.9 years ago by GenoMax

• written 5.9 years ago by nageshprabhu.k •

1 answer

As the amount of data increased it is possible that sequences added to the pool led to discovery of more duplicates (that were originally in separate lanes).

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written 5.9 years ago by GenoMax

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