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HTseq feature count handling of transcripts with shared exons?

I am curious to know how htseq will assign a read to a particular transcript if said read maps to a shared exon between two isoforms? If anyone has any experience with this or can offer any insight it would be greatly appreciated. Thank you in advance.

rna-seq htseq dge

1 answer

HTseq can only count at a gene level, not a transcript level, for precisely this reason.

Thank you, this is what I suspected but I wanted a second opinion!

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