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Convert .gbff (gene bank flat format) to gff3, gtf

As an example, I'm working with a reference genome for pineapple and need a GTF and GFF file. This is the assembly ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/902/162/155/GCA_902162155.2_F153.v0709/

All that's available is a .gbff file. I'm looking for a way to convert this into a gff3 and gtf file.

I am able to convert the gbff into a gff3 using bp_genbank2gff3

Using genome tools, I can validate this gff3 by running

gt gff3validator my.gff

and it tells me it's valid. But when i try and convert this gff to a gtf using either genome tools

(gt gff3_to_gtf my.gff)

or gffread

(gffread my.gff3 -T -o my.gtf)

it generates an empty file.

Am i going about the the right way?
Any help is appreciated

genome gtf gff gbff genebank

1 answer

It is not annotated, there is no gene model in the gbff file.

So essentially this is not possible? Is there any other way to generate a gtf in this case?

You can perform your own annotation, but it can be quite tough

Understood. Thank you very much for your answer!

Hi,

I am facing same problem. I have to perform RNA-seq analysis but I do not have reference gtf or gff file. How did you manage to convert gbff to gtf/gff ?

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