pcaExplorer greyed out should I wait or Is there a problem I have to fix
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3.7 years ago
roysense • 0
        #package upload

    library(cmapR)



    #object creation

    g<-GCT()

    gct_file<-system.file("extdata","PAAD_new.gct",package="cmapR")
    (g<-GCT(src=gct_file))

    class(g)



    #matrix Extraction m

    m<-mat(g)



    #column and row metadata extraction

    rdesc<-meta(g,dimension="row")

    cdesc<-meta(g,dimension="column")



    #column and row id extraction

    rid<-ids(g,dimension="row")

    cid<-ids(g,dimension="column")



    #Before using pcaExplorer we need two important files, the matrix file and the cdesc file

    write_tbl(m,"m.txt")

    write_tbl(cdesc,"cdesc.txt")

    #But we can directly attach the files to pcaExplorer
    library(pcaExplorer)
    pcaExplorer(countmatrix = m, coldata = cdesc)

In the pcaExplorer webpage, i pressed the generate dds and dst object and the screen greyed out

In the R console I am seeing Listening on http://127.0.0.1:6363

Warning: Error in DESeqDataSet: some values in assay are negative
  [No stack trace available]
Warning: Error in DESeqDataSet: some values in assay are negative
  [No stack trace available]
pcaExplorer RNA-Seq rna-seq PCA • 782 views
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Or should I remove negative values from the matrix?? But, if I do that will my results change?

P:S- All NaN values are already removed

Stars, Please help.

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What is the output of:

str(m)
summary(m)
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