Thank you for clarifying!
• 0 views
•
link
Can someone help clarify ways to measure coverage? Here is my current understanding, but I'm not sure about 2. 1. Library size is one way to measure coverage, and it is equal to 2. The number of reads that align to a particular gene. I hope this makes sense. Thanks!
Also, coverage (distinct from sequencing depth) estimates how many times each base in a genome or transcriptome is sequenced, on average. It's not a useful metric for RNA-seq because when a gene is highly expressed, it's going to have more sequencing reads than a lowly-expressed gene.
Thank you for clarifying!
Log in to answer this question.