This is a test version of Biostars. For the public version, visit https://www.biostars.org.
over-representation of sequences that map to "Drosophila melanogaster signal recognition particle

I found over-representation(count:114784, 1.65%, No Hit) of sequences that map to "Drosophila melanogaster signal recognition particle" but with my current knowledge, I am still unsure whether I should remove these reads from my TRAP-Seq data.

"signal recognition particle (SRP) controls the transport of secretory proteins into and across lipid bilayers"

https://blast.ncbi.nlm.nih.gov/Blast.cgi#alnHdr_1834199030

rna-seq trap-seq fastqc srp representation

Why do you want to remove some sequences? Without more details on the experiment and how the data was processed, it's impossible to answer.

The data is intact, I just ran FastQC on the fastqc files. I said I was unsure not that I want to remove the sequences. I was hoping someone could explain if this is a typical artifact of Ribo-Seq data (others have posted a similar question with no answers yet). Thanks for your comment though.

0 answers

No answers yet.

Log in to answer this question.