I have a protein sequence, which may be divided into different functional regions. I calculated the rate variation in different functional regions of the protein (e.g. 7/18 = 0.38, 282/597 = 0.47, 59/111 = 0.53, 16/30 = 0.53, 20/37 = 0.54, and 11/30 = 0.36). I want to check if the rate variation among different regions are significant. Can I use one way Anova to do it ? IS their any online tool or software available that will do the test. Suggestions will be highly appreciated.